TY - CHAP
T1 - Identification of Antibody Biomarker Using High-Density Nucleic Acid Programmable Protein Array
AU - Song, Lusheng
AU - Wiktor, Peter
AU - Qiu, Ji
AU - LaBaer, Joshua
N1 - Publisher Copyright:
© 2021, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2021
Y1 - 2021
N2 - A novel protein microarray technology, called high-density nucleic acid programmable protein array (HD-NAPPA), enables the serological screening of thousands of proteins at one time. HD-NAPPA extends the capabilities of NAPPA, which produces protein microarrays on a conventional glass microscope slide. By comparison, HD-NAPPA displays proteins in over 10,000 nanowells etched in a silicon slide. Proteins on HD-NAPPA are expressed in the individual isolated nanowells, via in vitro transcription and translation (IVTT), without any diffusion during incubation. Here we describe the method for antibody biomarker identification using HD-NAPPA, including four main steps: (1) HD-NAPPA array protein expression, (2) primary antibodies (serum/plasma) probing, (3) secondary antibody visualization, and (4) image scanning and data processing.
AB - A novel protein microarray technology, called high-density nucleic acid programmable protein array (HD-NAPPA), enables the serological screening of thousands of proteins at one time. HD-NAPPA extends the capabilities of NAPPA, which produces protein microarrays on a conventional glass microscope slide. By comparison, HD-NAPPA displays proteins in over 10,000 nanowells etched in a silicon slide. Proteins on HD-NAPPA are expressed in the individual isolated nanowells, via in vitro transcription and translation (IVTT), without any diffusion during incubation. Here we describe the method for antibody biomarker identification using HD-NAPPA, including four main steps: (1) HD-NAPPA array protein expression, (2) primary antibodies (serum/plasma) probing, (3) secondary antibody visualization, and (4) image scanning and data processing.
KW - Antibody biomarker discovery
KW - Biomarker
KW - HD-NAPPA
KW - Nucleic acid programmable protein array
KW - Silicon nanowells
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U2 - 10.1007/978-1-0716-1562-1_4
DO - 10.1007/978-1-0716-1562-1_4
M3 - Chapter
C2 - 34115351
AN - SCOPUS:85108242096
T3 - Methods in Molecular Biology
SP - 47
EP - 64
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -