Homeostatic regulation of spontaneous and evoked synaptic transmission in two steps

Richard Gerkin, David W. Nauen, Fang Xu, Guo Qiang Bi

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Background: During development both Hebbian and homeostatic mechanisms regulate synaptic efficacy, usually working in opposite directions in response to neuronal activity. Homeostatic plasticity has often been investigated by assaying changes in spontaneous synaptic transmission resulting from chronic circuit inactivation. However, effects of inactivation on evoked transmission have been less frequently reported. Importantly, contributions from the effects of circuit inactivation and reactivation on synaptic efficacy have not been individuated. Results: Here we show for developing hippocampal neurons in primary culture that chronic inactivation with TTX results in increased mean amplitude of miniature synaptic currents (mEPSCs), but not evoked synaptic currents (eEPSCs). However, changes in quantal properties of transmission, partially reflected in mEPSCs, accurately predicted higher-order statistical properties of eEPSCs. The classical prediction of homeostasis - increased strength of evoked transmission - was realized after explicit circuit reactivation, in the form of cells' pairwise connection probability. In contrast, distributions of eEPSC amplitudes for control and inactivated-then- reactivated groups matched throughout. Conclusions: Homeostatic up-regulation of evoked synaptic transmission in developing hippocampal neurons in primary culture requires both the inactivation and reactivation stages, leading to a net increase in functional circuit connectivity.

Original languageEnglish (US)
Article number38
JournalMolecular Brain
Issue number1
StatePublished - 2013


  • Homeostasis
  • Metaplasticity
  • Quantal hypothesis

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience


Dive into the research topics of 'Homeostatic regulation of spontaneous and evoked synaptic transmission in two steps'. Together they form a unique fingerprint.

Cite this