Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome

Wei Sun; Shifeng Wang; Roy Curtiss

doi:10.1128/AEM.00940-08

Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome

Wei Sun
, Shifeng Wang
, Roy Curtiss

Life Sciences, School of (SOLS)

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

An efficient two-step recombination method for markerless gene deletion and insertion that can be used for repetitive genetic modification in Yersinia pestis was developed. The method combines γ Red recombination and counterselective screening (sacB gene) and can be used for genetic modification of Y. pestis to construct live attenuated vaccines.

Original language	English (US)
Pages (from-to)	4241-4245
Number of pages	5
Journal	Applied and environmental microbiology
Volume	74
Issue number	13
DOIs	https://doi.org/10.1128/AEM.00940-08
State	Published - Jul 2008

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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10.1128/AEM.00940-08

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abstract = "An efficient two-step recombination method for markerless gene deletion and insertion that can be used for repetitive genetic modification in Yersinia pestis was developed. The method combines γ Red recombination and counterselective screening (sacB gene) and can be used for genetic modification of Y. pestis to construct live attenuated vaccines.",

author = "Wei Sun and Shifeng Wang and Roy Curtiss",

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AB - An efficient two-step recombination method for markerless gene deletion and insertion that can be used for repetitive genetic modification in Yersinia pestis was developed. The method combines γ Red recombination and counterselective screening (sacB gene) and can be used for genetic modification of Y. pestis to construct live attenuated vaccines.

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Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome

Abstract

ASJC Scopus subject areas

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