Global DNA hypomethylation is associated with in utero exposure to cotinine and perfluorinated alkyl compounds

Rafael Guerrero-Preston, Lynn R. Goldman, Priscilla Brebi-Mieville, Carmen Ili-Gangas, Cynthia LeBron, Mireya Hernández-Arroyo, Frank R. Witter, Ben J. Apelberg, Marina Roystacher, Andrew Jaffe, Rolf Halden, David Sidransky

Research output: Contribution to journalArticlepeer-review

162 Scopus citations


Environmental exposures in utero may alter the epigenome, thus impacting chromosomal stability and gene expression. We hypothesized that in utero exposures to maternal smoking and perfluoroalkyl compounds (PFCs) are associated with global DNA hypomethylation in umbilical cord serum. Our objective was to determine if global DNA methylation could be used as a biomarker of in utero exposures to maternal smoking and PFCs. Using an ELISA-based method, global DNA methylation was quantified in umbilical cord serum from 30 newborns with high (>10 ng/ml, mean 123.8 ng/ml), low (range 1-10 ng/ml, mean 1.6 ng/ml) and very low (<1 ng/ml, mean 0.06 ng/ml) cord serum cotinine levels. Y chromosome analysis was performed to rule out maternal DNA cross-contamination. Cord serum global DNA methylation showed an inverse dose response to serum cotinine levels (p < 0.001). Global DNA methylation levels in cord blood were the lowest among newborns with smoking mothers (mean = 15.04%; 95% CI, 8.4, 21.7) when compared to babies of mothers who were second-hand smokers (21.1%; 95% CI, 16.6, 25.5) and non-smokers (mean = 29.2%; 95% CI, 20.1, 38.1). Global DNA methylation was inversely correlated with serum PFOA (r = -0.35, p = 0.06) but not PFOS levels. Serum Y chromosome analyses did not detect maternal DNA cross-contamination. This study supports the use of global DNA methylation status as a biomarker of in utero exposure to cigarette smoke and PFCs.

Original languageEnglish (US)
Pages (from-to)539-546
Number of pages8
Issue number6
StatePublished - Aug 2010


  • Cigarette smoke
  • Epigenomics
  • Global DNA methylation
  • Hypomethylation
  • Perfluorooctane sulfonate
  • Perfluorooctanoate
  • Umbilical cord serum

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research


Dive into the research topics of 'Global DNA hypomethylation is associated with in utero exposure to cotinine and perfluorinated alkyl compounds'. Together they form a unique fingerprint.

Cite this