Glial cell and fibroblast cytotoxicity study on 4026-cyclotene photosensitive benzocyclobutene (BCB) polymer films

Gholamreza Ehteshami, Amarjit Singh, Gene Coryell, Stephen Massia, Jiping He, Gregory Raupp

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Photosensitive benzocyclobutene (photo-BCB) is a class of polymers with the trade name Cyclotene™. The photoimagable property of Cyclotene™ makes it suitable for the manufacture of microelectronic devices. The motivation behind this study is that we see an exciting application of photo-BCB as substrates in implantable microelectronic biomedical devices due to several desirable properties distinctive from other polymer materials. To our knowledge, however, photo-BCB has never been tested for biomedical implant applications, as evidenced by the lack reported data on its biocompatibility. This study takes the first step towards assessing photo-BCB biocompatibility by evaluating the cytotoxicity and cell adhesion behavior of Cyclotene 4026™ coatings exposed to monolayers of glial and fibroblast cells in vitro. It can be concluded from these studies that photo-BCB films deposited on silicon wafers using microfabrication processes did not adversely affect 3T3 fibroblast and T98-G glial cell function in vitro. We also successfully rendered photo-BCB films non-adhesive (no significant fibroblast or glial cell adhesion) with surface immobilized dextran using methods developed for other biomaterials and applications. Future work will further develop prototype photo-BCB microelectrode devices for chronic neural implant applications.

Original languageEnglish (US)
Pages (from-to)1105-1116
Number of pages12
JournalJournal of Biomaterials Science, Polymer Edition
Issue number10
StatePublished - Nov 14 2003


  • Cell-resistant surface
  • Immobilized dextran
  • Neural prosthetics
  • Photosensitive benzocyclobutene

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering


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