TY - JOUR
T1 - Enucleation of mammalian cells by cytochalasin B. I. Characterization of anucleate cells
AU - Poste, G.
N1 - Funding Information:
This work was supported by grants from the Medical ResearchC ouncil, The Animal Health Trust, National Fund for Research in Crippling Diseases (Polio Research Fund) and the Cancer Research Campaign.
PY - 1972/8
Y1 - 1972/8
N2 - Cytochalasin B, a metabolite isolated from cultures of the fungus Helminthosporium dematioideum, induced enucleation of a variety of mammalian cells in vitro when used at concentrations of 30 gmg/ml or greater. The susceptibility of different cell types to enucleation by cytochalasin varied significantly. Murine cells, notably the L929 line and peritoneal macrophages, were most susceptible to enucleation but anucleate cells were also obtained routinely from certain human and hamster cell cultures. The effect of different culture conditions on the number and viability of anucleate cells was investigated. Anucleate cells obtained by this method were viable for 24-36 h and were able to synthesize protein. The possible mechanism of cytochalasin B-induced enucleation was discussed.
AB - Cytochalasin B, a metabolite isolated from cultures of the fungus Helminthosporium dematioideum, induced enucleation of a variety of mammalian cells in vitro when used at concentrations of 30 gmg/ml or greater. The susceptibility of different cell types to enucleation by cytochalasin varied significantly. Murine cells, notably the L929 line and peritoneal macrophages, were most susceptible to enucleation but anucleate cells were also obtained routinely from certain human and hamster cell cultures. The effect of different culture conditions on the number and viability of anucleate cells was investigated. Anucleate cells obtained by this method were viable for 24-36 h and were able to synthesize protein. The possible mechanism of cytochalasin B-induced enucleation was discussed.
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U2 - 10.1016/0014-4827(72)90049-3
DO - 10.1016/0014-4827(72)90049-3
M3 - Article
C2 - 4626574
AN - SCOPUS:0015385579
SN - 0014-4827
VL - 73
SP - 273
EP - 286
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -