Emerging tools for real-time label-free detection of interactions on functional protein microarrays

Niroshan Ramachandran, Dale N. Larson, Peter R.H. Stark, Eugenie Hainsworth, Joshua LaBaer

Research output: Contribution to journalArticlepeer-review

146 Scopus citations


The availability of extensive genomic information and content has spawned an era of high-throughput screening that is generating large sets of functional genomic data. In particular, the need to understand the biochemical wiring within a cell has introduced novel approaches to map the intricate networks of biological interactions arising from the interactions of proteins. The current technologies for assaying protein interactions - yeast two-hybrid and immunoprecipitation with mass spectrometric detection - have met with considerable success. However, the parallel use of these approaches has identified only a small fraction of physiologically relevant interactions among proteins, neglecting all nonprotein interactions, such as with metabolites, lipids, DNA and small molecules. This highlights the need for further development of proteome scale technologies that enable the study of protein function. Here we discuss recent advances in high-throughput technologies for displaying proteins on functional protein microarrays and the real-time label-free detection of interactions using probes of the local index of refraction, carbon nanotubes and nanowires, or microelectromechanical systems cantilevers. The combination of these technologies will facilitate the large-scale study of protein interactions with proteins as well as with other biomolecules.

Original languageEnglish (US)
Pages (from-to)5412-5425
Number of pages14
JournalFEBS Journal
Issue number21
StatePublished - Nov 2005
Externally publishedYes


  • Carbon nanowires
  • Cell-free system
  • Colorimetric resonant reflection
  • Label-free detection
  • MEMS cantilevers
  • Nanohole array sensors
  • Protein interactions
  • Protein microarrays
  • Protein purification
  • Self-assembling protein arrays
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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