TY - JOUR
T1 - Electrospray ionization and matrix-assisted laser desorption ionization mass spectrometry - Emerging technologies in biomedical sciences
AU - Bakhtiar, Ray
AU - Nelson, Randall W.
N1 - Funding Information:
Drs. Jennifer R. Krone, Kemmons A. Tubbs, and Dobrin Nedelkov (Intrinsic Bioprobes, Inc., Tempe, AZ) are acknowledged for their contributions to BIA/MS. This publication was supported, in part, by Grant 1R43 CA82079–01 from the National Cancer Institute (R. W. N.). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the National Cancer Institute. In addition, R. B. thanks Professors G. Siuzdak (The Scripps Research Institute), R. Aebersold (University of Washington), C. V. Robinson (University of Oxford-U.K.), W. Trager (University of Washington), C. Obinger (University of Agricultural Sciences, Vienna, Austria), P. Limbach (Louisiana State University), A. Burlingame (University of California at San Francisco), and Drs. L. Koenigs (University of California at San Francisco) and D. Figeys (National Research Council-Canada) for providing some of the figures and manuscript reprints.
PY - 2000/4/15
Y1 - 2000/4/15
N2 - Tremendous progress in biomedical sciences has been made possible in part by recent advances in bioanalytical methods, in particular biological mass spectrometry. Since the introduction of electrospray ionization mass spectrometry (ESI-MS) in 1984 and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) in 1988, the field of bioanalytical mass spectrometry has seen rapid growth. In concert with separation techniques such as capillary electrophoresis and high performance liquid chromatography, mass spectrometry allows characterization of a large array of small organic molecules, peptides, proteins, oligonucleotides, and RNA fragments. Thus, substantially more expedient and definitive determination of molecular weight is now possible by mass spectrometric analysis. In this commentary, general descriptions of ESI- and MALDI-MS are presented. Furthermore, several recent developments and applications in addressing difficult biological problems are discussed. (C) 2000 Elsevier Science Inc.
AB - Tremendous progress in biomedical sciences has been made possible in part by recent advances in bioanalytical methods, in particular biological mass spectrometry. Since the introduction of electrospray ionization mass spectrometry (ESI-MS) in 1984 and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) in 1988, the field of bioanalytical mass spectrometry has seen rapid growth. In concert with separation techniques such as capillary electrophoresis and high performance liquid chromatography, mass spectrometry allows characterization of a large array of small organic molecules, peptides, proteins, oligonucleotides, and RNA fragments. Thus, substantially more expedient and definitive determination of molecular weight is now possible by mass spectrometric analysis. In this commentary, general descriptions of ESI- and MALDI-MS are presented. Furthermore, several recent developments and applications in addressing difficult biological problems are discussed. (C) 2000 Elsevier Science Inc.
KW - Biomolecular interaction analysis
KW - Combinatorial chemistry
KW - High throughput mass spectrometry
KW - Non-covalent complexes
KW - Proteomics
KW - Sequencing
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U2 - 10.1016/S0006-2952(99)00317-2
DO - 10.1016/S0006-2952(99)00317-2
M3 - Article
C2 - 10692554
AN - SCOPUS:0033955248
SN - 0006-2952
VL - 59
SP - 891
EP - 905
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
IS - 8
ER -