Electrophoretic Deformation of Individual Transfer RNA Molecules Reveals Their Identity

Robert Y. Henley, Brian Alan Ashcroft, Ian Farrell, Barry S. Cooperman, Stuart Lindsay, Meni Wanunu

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


It has been hypothesized that the ribosome gains additional fidelity during protein translation by probing structural differences in tRNA species. We measure the translocation kinetics of different tRNA species through ∼3 nm diameter synthetic nanopores. Each tRNA species varies in the time scale with which it is deformed from equilibrium, as in the translocation step of protein translation. Using machine-learning algorithms, we can differentiate among five tRNA species, analyze the ratios of tRNA binary mixtures, and distinguish tRNA isoacceptors. (Figure Presented).

Original languageEnglish (US)
Pages (from-to)138-144
Number of pages7
JournalNano Letters
Issue number1
StatePublished - Jan 13 2016


  • RNA
  • SVM
  • nanopores
  • single molecule
  • tRNA

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering


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