TY - GEN
T1 - Electronically Stimulated Segmented Flow for Reduced Sample Consumption During Serial Femtosecond Crystallography
AU - Doppler, Diandra
AU - Sonker, Mukul
AU - Egatz-Gomez, Ana
AU - Nelson, Garrett
AU - Rabbani, Mohammad Towshif
AU - Villarreal, Jorvani Cruz
AU - Nazari, Reza
AU - Zaare, Sahba
AU - Thifault, Darren
AU - Botha, Sabine
AU - Grant, Thomas
AU - Fromme, Petra
AU - Kirian, Richard
AU - Ros, Alexandra
N1 - Funding Information:
Funding Agencies: NIH-(No. R01GM095583, No. 1S10OD021816-01), NSF-(No. 1565180), BioXFEL- (No. 1231306)
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) is an emerging and growing field in structural discovery; one of its principal achievements is the ability to resolve the structure of intricate membrane proteins at room temperature. Because all XFELs currently operational are pulsed, up to 99% of the sample delivered to the X-ray beam during its 'off-time' is wasted. To solve this major problem of large sample consumption, we report a revolutionary sample saving injection method which is compatible with all current XFELs.
AB - Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) is an emerging and growing field in structural discovery; one of its principal achievements is the ability to resolve the structure of intricate membrane proteins at room temperature. Because all XFELs currently operational are pulsed, up to 99% of the sample delivered to the X-ray beam during its 'off-time' is wasted. To solve this major problem of large sample consumption, we report a revolutionary sample saving injection method which is compatible with all current XFELs.
KW - 3D-printing
KW - microfluidics
KW - sample conservation
KW - serial femtosecond crystallography
UR - http://www.scopus.com/inward/record.url?scp=85136980055&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85136980055&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136980055
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 997
EP - 998
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -