Electron-transfer properties of cytochrome c Langmuir-Blodgett films and interactions of cytochrome c with lipids

S. Boussaad; L. Dziri; R. Arechabaleta; N. J. Tao; R. M. Leblanc

doi:10.1021/la980319l

Electron-transfer properties of cytochrome c Langmuir-Blodgett films and interactions of cytochrome c with lipids

S. Boussaad, L. Dziri, R. Arechabaleta, N. J. Tao, R. M. Leblanc

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Abstract

The structural and electron-transfer properties of cytochrome c (Cyt c) Langmuir-Blodgett (LB) films have been studied on graphite electrode with tapping mode atomic force microscopy and cyclic voltammetry (CV). Cyt c in the LB films forms an ordered monolayer in which the individual proteins pack into a quasi-hexagonal structure. The monolayer undergoes a reversible electron-transfer reaction in phosphate buffer. The interactions of Cyt c with cardiolipin (CL) and phosphatidylcholine (PC) LB films have been studied. The LB films of CL and PC are both ordered on graphite, but their interactions with Cyt c are quite different. On a CL monolayer, Cyt c adsorbs spontaneously and the adsorbed protein preserves the electron-transfer reaction. However, on a PC monolayer, Cyt c does not adsorb.

Original language	English (US)
Pages (from-to)	6215-6219
Number of pages	5
Journal	Langmuir
Volume	14
Issue number	21
DOIs	https://doi.org/10.1021/la980319l
State	Published - Oct 13 1998

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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title = "Electron-transfer properties of cytochrome c Langmuir-Blodgett films and interactions of cytochrome c with lipids",

abstract = "The structural and electron-transfer properties of cytochrome c (Cyt c) Langmuir-Blodgett (LB) films have been studied on graphite electrode with tapping mode atomic force microscopy and cyclic voltammetry (CV). Cyt c in the LB films forms an ordered monolayer in which the individual proteins pack into a quasi-hexagonal structure. The monolayer undergoes a reversible electron-transfer reaction in phosphate buffer. The interactions of Cyt c with cardiolipin (CL) and phosphatidylcholine (PC) LB films have been studied. The LB films of CL and PC are both ordered on graphite, but their interactions with Cyt c are quite different. On a CL monolayer, Cyt c adsorbs spontaneously and the adsorbed protein preserves the electron-transfer reaction. However, on a PC monolayer, Cyt c does not adsorb.",

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T1 - Electron-transfer properties of cytochrome c Langmuir-Blodgett films and interactions of cytochrome c with lipids

AU - Boussaad, S.

AU - Dziri, L.

AU - Arechabaleta, R.

AU - Tao, N. J.

AU - Leblanc, R. M.

PY - 1998/10/13

Y1 - 1998/10/13

N2 - The structural and electron-transfer properties of cytochrome c (Cyt c) Langmuir-Blodgett (LB) films have been studied on graphite electrode with tapping mode atomic force microscopy and cyclic voltammetry (CV). Cyt c in the LB films forms an ordered monolayer in which the individual proteins pack into a quasi-hexagonal structure. The monolayer undergoes a reversible electron-transfer reaction in phosphate buffer. The interactions of Cyt c with cardiolipin (CL) and phosphatidylcholine (PC) LB films have been studied. The LB films of CL and PC are both ordered on graphite, but their interactions with Cyt c are quite different. On a CL monolayer, Cyt c adsorbs spontaneously and the adsorbed protein preserves the electron-transfer reaction. However, on a PC monolayer, Cyt c does not adsorb.

AB - The structural and electron-transfer properties of cytochrome c (Cyt c) Langmuir-Blodgett (LB) films have been studied on graphite electrode with tapping mode atomic force microscopy and cyclic voltammetry (CV). Cyt c in the LB films forms an ordered monolayer in which the individual proteins pack into a quasi-hexagonal structure. The monolayer undergoes a reversible electron-transfer reaction in phosphate buffer. The interactions of Cyt c with cardiolipin (CL) and phosphatidylcholine (PC) LB films have been studied. The LB films of CL and PC are both ordered on graphite, but their interactions with Cyt c are quite different. On a CL monolayer, Cyt c adsorbs spontaneously and the adsorbed protein preserves the electron-transfer reaction. However, on a PC monolayer, Cyt c does not adsorb.

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Electron-transfer properties of cytochrome c Langmuir-Blodgett films and interactions of cytochrome c with lipids

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