TY - JOUR
T1 - Effective inhibition of influenza virus production in cultured cells by external guide sequences and ribonuclease P
AU - Plehn-Dujowich, Debora
AU - Altman, Sidney
PY - 1998/6/23
Y1 - 1998/6/23
N2 - The polymerase (PB2) and nucleocapsid (NP) genes encoded by the genome of influenza virus are essential for replication of the virus. When synthetic genes that express RNAs for external guide sequences targeted to the mRNAs of the PB2 and NP genes are stably incorporated into mouse cells in tissue culture, infection of these cells with influenza virus is nonproductive. Endogenous RNase P cleaves the targeted influenza virus mRNAs when they are in a complex with the external guide sequences. Targeting two different mRNAs simultaneously inhibits viral particle production more efficiently than does targeting only one mRNA.
AB - The polymerase (PB2) and nucleocapsid (NP) genes encoded by the genome of influenza virus are essential for replication of the virus. When synthetic genes that express RNAs for external guide sequences targeted to the mRNAs of the PB2 and NP genes are stably incorporated into mouse cells in tissue culture, infection of these cells with influenza virus is nonproductive. Endogenous RNase P cleaves the targeted influenza virus mRNAs when they are in a complex with the external guide sequences. Targeting two different mRNAs simultaneously inhibits viral particle production more efficiently than does targeting only one mRNA.
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U2 - 10.1073/pnas.95.13.7327
DO - 10.1073/pnas.95.13.7327
M3 - Article
C2 - 9636148
AN - SCOPUS:0032560566
SN - 0027-8424
VL - 95
SP - 7327
EP - 7332
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 13
ER -