TY - JOUR
T1 - Effect of cadmium accumulation on green algae Chlamydomonas reinhardtii and acid-tolerant Chlamydomonas CPCC 121
AU - Samadani, Mahshid
AU - Perreault, Francois
AU - Oukarroum, Abdallah
AU - Dewez, David
N1 - Funding Information:
This research was financially supported in part by the Department of Chemistry and the Faculty of Sciences of the University of Quebec in Montreal (UQÀM) and by the Individual Discovery Grant awarded to D. Dewez from the Natural Sciences and Engineering Research Council of Canada (NSERC , RGPIN 415572-2013 ). Authors wish to thank the staff of the Electron Microscopy Research Facility at McGill University (Montreal, Canada).
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2018/1
Y1 - 2018/1
N2 - Cadmium is one of the most dangerous metals found in wastewater since exposure to low concentrations are highly toxic for cellular functions. In this study, the effect of cadmium accumulation on Chlamydomonas reinhardtii and acid-tolerant strain CPCC 121 was investigated during 48 h under 100–600 μM of Cd and two pH conditions (4 and 7). The toxicity of accumulated Cd was determined by the change of cellular and photosynthetic parameters. Obtained results showed that the maximum capacity of Cd accumulation in algal biomass was reached for both strains at 24 h of exposure to 600 μM of Cd. Under this condition, C. reinhardtii showed a higher uptake of Cd compared to the strain CPCC 121, inducing a stronger cellular toxic impact. Chlamydomonas CPCC 121 showed a tolerance for Cd due to the exclusion of Cd at the cell wall surface, which was higher at pH 4 than pH 7. TEM images and EDX spectrum of Cd distribution within the cell confirmed the role of the cell wall as a barrier for Cd uptake. Although Cd2+ concentration was the highest in the medium, CPCC 121 was the most tolerant at pH 4, but was not enough efficient to be considered for the phycoremediation of Cd. At neutral pH, the efficiency of C. reinhardtii for the removal of Cd was limited by its toxicity, which was dependent to the concentration of Cd in the medium and the time of exposure.
AB - Cadmium is one of the most dangerous metals found in wastewater since exposure to low concentrations are highly toxic for cellular functions. In this study, the effect of cadmium accumulation on Chlamydomonas reinhardtii and acid-tolerant strain CPCC 121 was investigated during 48 h under 100–600 μM of Cd and two pH conditions (4 and 7). The toxicity of accumulated Cd was determined by the change of cellular and photosynthetic parameters. Obtained results showed that the maximum capacity of Cd accumulation in algal biomass was reached for both strains at 24 h of exposure to 600 μM of Cd. Under this condition, C. reinhardtii showed a higher uptake of Cd compared to the strain CPCC 121, inducing a stronger cellular toxic impact. Chlamydomonas CPCC 121 showed a tolerance for Cd due to the exclusion of Cd at the cell wall surface, which was higher at pH 4 than pH 7. TEM images and EDX spectrum of Cd distribution within the cell confirmed the role of the cell wall as a barrier for Cd uptake. Although Cd2+ concentration was the highest in the medium, CPCC 121 was the most tolerant at pH 4, but was not enough efficient to be considered for the phycoremediation of Cd. At neutral pH, the efficiency of C. reinhardtii for the removal of Cd was limited by its toxicity, which was dependent to the concentration of Cd in the medium and the time of exposure.
KW - Cadmium accumulation
KW - Chlamydomonas CPCC 121
KW - Chlamydomonas reinhardtii
KW - Green algae
KW - Tolerance
KW - Toxicity
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U2 - 10.1016/j.chemosphere.2017.10.017
DO - 10.1016/j.chemosphere.2017.10.017
M3 - Article
C2 - 29032262
AN - SCOPUS:85034577787
SN - 0045-6535
VL - 191
SP - 174
EP - 182
JO - Chemosphere
JF - Chemosphere
ER -