TY - JOUR
T1 - DNA origami
AU - Dey, Swarup
AU - Fan, Chunhai
AU - Gothelf, Kurt V.
AU - Li, Jiang
AU - Lin, Chenxiang
AU - Liu, Longfei
AU - Liu, Na
AU - Nijenhuis, Minke A.D.
AU - Saccà, Barbara
AU - Simmel, Friedrich C.
AU - Yan, Hao
AU - Zhan, Pengfei
N1 - Funding Information:
C.F. and J.L. were supported by the National Natural Science Foundation of China (21991134, 21834007) and the Shanghai Municipal Science and Technology Commission (19JC1410300). K.V.G. and M.A.D.N. were supported by DNA-Based Modular Nanorobotics (DNA-Robotics) and the Marie Curie Innovative Training Network (MRC ITN) under EU H2020 (Project ID: 765703). P.Z. and N.L. were supported by a European Research Council (ERC Dynamic Nano) grant. B.S. was supported by the Deutsche Forschungsgemeinschaft (CRC-1093).
Publisher Copyright:
© 2021, Springer Nature Limited.
PY - 2021/12
Y1 - 2021/12
N2 - Biological materials are self-assembled with near-atomic precision in living cells, whereas synthetic 3D structures generally lack such precision and controllability. Recently, DNA nanotechnology, especially DNA origami technology, has been useful in the bottom-up fabrication of well-defined nanostructures ranging from tens of nanometres to sub-micrometres. In this Primer, we summarize the methodologies of DNA origami technology, including origami design, synthesis, functionalization and characterization. We highlight applications of origami structures in nanofabrication, nanophotonics and nanoelectronics, catalysis, computation, molecular machines, bioimaging, drug delivery and biophysics. We identify challenges for the field, including size limits, stability issues and the scale of production, and discuss their possible solutions. We further provide an outlook on next-generation DNA origami techniques that will allow in vivo synthesis and multiscale manufacturing.
AB - Biological materials are self-assembled with near-atomic precision in living cells, whereas synthetic 3D structures generally lack such precision and controllability. Recently, DNA nanotechnology, especially DNA origami technology, has been useful in the bottom-up fabrication of well-defined nanostructures ranging from tens of nanometres to sub-micrometres. In this Primer, we summarize the methodologies of DNA origami technology, including origami design, synthesis, functionalization and characterization. We highlight applications of origami structures in nanofabrication, nanophotonics and nanoelectronics, catalysis, computation, molecular machines, bioimaging, drug delivery and biophysics. We identify challenges for the field, including size limits, stability issues and the scale of production, and discuss their possible solutions. We further provide an outlook on next-generation DNA origami techniques that will allow in vivo synthesis and multiscale manufacturing.
UR - http://www.scopus.com/inward/record.url?scp=85130448382&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85130448382&partnerID=8YFLogxK
U2 - 10.1038/s43586-020-00009-8
DO - 10.1038/s43586-020-00009-8
M3 - Review article
AN - SCOPUS:85130448382
SN - 2662-8449
VL - 1
JO - Nature Reviews Methods Primers
JF - Nature Reviews Methods Primers
IS - 1
M1 - 13
ER -