TY - JOUR
T1 - Differential effects of mutations in the protein and RNA moieties of RNase P on the efficiency of suppression by various tRNA suppressors
AU - Kirsebom, Leif A.
AU - Baer, Madeline F.
AU - Altman, Sidney
N1 - Funding Information:
This work was supported by grants to Sidney Altman from the National Institute of Health and the National Science Foundation of the United States. Dr T. Ruusala is acknowledged for helpful comments on the manuscript. L.A.K. is a fellow of the Swedish Research Council.
PY - 1988/12/20
Y1 - 1988/12/20
N2 - We have studied the efficiency of suppression by tRNA suppressors in vivo in strains of Escherichia coli that harbor a mutation in the rnpA gene, the gene for the protein component (C5) of RNase P, and in strains that carry several different alleles of the rnpB gene, the gene for the RNA component (M1) of RNase P. Depending on the genetic background, different efficiencies of suppression by the various tRNA suppressors were observed. Thus, mutations in rnpA have separable and distinct effects from mutations in rnpB on the processing of tRNA precursors by RNase P. In addition, the efficiency of suppression by several derivatives of E. coli tRNATyr Su3 changed as the genetic background was altered.
AB - We have studied the efficiency of suppression by tRNA suppressors in vivo in strains of Escherichia coli that harbor a mutation in the rnpA gene, the gene for the protein component (C5) of RNase P, and in strains that carry several different alleles of the rnpB gene, the gene for the RNA component (M1) of RNase P. Depending on the genetic background, different efficiencies of suppression by the various tRNA suppressors were observed. Thus, mutations in rnpA have separable and distinct effects from mutations in rnpB on the processing of tRNA precursors by RNase P. In addition, the efficiency of suppression by several derivatives of E. coli tRNATyr Su3 changed as the genetic background was altered.
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U2 - 10.1016/0022-2836(88)90048-4
DO - 10.1016/0022-2836(88)90048-4
M3 - Article
C2 - 2464697
AN - SCOPUS:0024206114
SN - 0022-2836
VL - 204
SP - 879
EP - 888
JO - Journal of molecular biology
JF - Journal of molecular biology
IS - 4
ER -