Development of the resolution theory for electrophoretic exclusion

Stacy M. Kenyon, Michael W. Keebaugh, Mark Hayes

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Electrophoretic exclusion, a technique that differentiates species in bulk solution near a channel entrance, has been demonstrated on benchtop and microdevice designs. In these systems, separation occurs when the electrophoretic velocity of one species is greater than the opposing hydrodynamic flow, while the velocity of the other species is less than that flow. Although exclusion has been demonstrated in multiple systems for a range of analytes, a theoretical assessment of resolution has not been addressed. To compare the results of these calculations to traditional techniques, the performance is expressed in terms of smallest difference in electrophoretic mobilities that can be completely separated (R = 1.5). The calculations indicate that closest resolvable species (Δμmin) differ by approximately 10-13 m2/Vs and peak capacity (nc) is 1000. Published experimental data were compared to these calculated results.

Original languageEnglish (US)
Pages (from-to)2551-2559
Number of pages9
Issue number18
StatePublished - Sep 2014


  • Electrophoretic exclusion
  • Resolution
  • Separation science

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry


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