Detection of calcium binding by photosystem II polypeptides immobilised onto nitrocellulose membrane

Andrew N. Webber; John C. Gray

doi:10.1016/0014-5793(89)80020-1

Detection of calcium binding by photosystem II polypeptides immobilised onto nitrocellulose membrane

Andrew N. Webber, John C. Gray

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Photosystem II calcium-binding polypeptides have been detected by their ability to selectively bind ⁴⁵Ca when immobilised onto nitrocellulose membrane following SDS-polyacrylamide gel electrophoresis. Two calcium-binding polypeptides of 26 kDa and 24 kDa are shown to be components of LHCII. The 24 kDa polypeptide was further characterised by N-terminal amino acid sequence analysis and shown to be the product of a type II cab gene. A third polypeptide of 33 kDa bound calcium more weakly and was not positively identified.

Original language	English (US)
Pages (from-to)	79-82
Number of pages	4
Journal	FEBS Letters
Volume	249
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(89)80020-1
State	Published - May 22 1989
Externally published	Yes

Keywords

Calcium-binding polypeptide
Light-harvesting complex II
N-terminal sequence
Photosystem II

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(89)80020-1

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title = "Detection of calcium binding by photosystem II polypeptides immobilised onto nitrocellulose membrane",

abstract = "Photosystem II calcium-binding polypeptides have been detected by their ability to selectively bind 45Ca when immobilised onto nitrocellulose membrane following SDS-polyacrylamide gel electrophoresis. Two calcium-binding polypeptides of 26 kDa and 24 kDa are shown to be components of LHCII. The 24 kDa polypeptide was further characterised by N-terminal amino acid sequence analysis and shown to be the product of a type II cab gene. A third polypeptide of 33 kDa bound calcium more weakly and was not positively identified.",

keywords = "Calcium-binding polypeptide, Light-harvesting complex II, N-terminal sequence, Photosystem II",

author = "Webber, {Andrew N.} and Gray, {John C.}",

note = "Funding Information: AcknowledgementWs:e thank Dr L. Packmanf or aminoa cid sequence analysis. A.N.W. was an SERC Postdoctoral ResearchF ellow. This work was supportedb y grants from SERC and the Nuffield Foundation.",

year = "1989",

month = may,

day = "22",

doi = "10.1016/0014-5793(89)80020-1",

language = "English (US)",

volume = "249",

pages = "79--82",

journal = "FEBS Letters",

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number = "1",

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T1 - Detection of calcium binding by photosystem II polypeptides immobilised onto nitrocellulose membrane

AU - Webber, Andrew N.

AU - Gray, John C.

N1 - Funding Information: AcknowledgementWs:e thank Dr L. Packmanf or aminoa cid sequence analysis. A.N.W. was an SERC Postdoctoral ResearchF ellow. This work was supportedb y grants from SERC and the Nuffield Foundation.

PY - 1989/5/22

Y1 - 1989/5/22

N2 - Photosystem II calcium-binding polypeptides have been detected by their ability to selectively bind 45Ca when immobilised onto nitrocellulose membrane following SDS-polyacrylamide gel electrophoresis. Two calcium-binding polypeptides of 26 kDa and 24 kDa are shown to be components of LHCII. The 24 kDa polypeptide was further characterised by N-terminal amino acid sequence analysis and shown to be the product of a type II cab gene. A third polypeptide of 33 kDa bound calcium more weakly and was not positively identified.

AB - Photosystem II calcium-binding polypeptides have been detected by their ability to selectively bind 45Ca when immobilised onto nitrocellulose membrane following SDS-polyacrylamide gel electrophoresis. Two calcium-binding polypeptides of 26 kDa and 24 kDa are shown to be components of LHCII. The 24 kDa polypeptide was further characterised by N-terminal amino acid sequence analysis and shown to be the product of a type II cab gene. A third polypeptide of 33 kDa bound calcium more weakly and was not positively identified.

KW - Calcium-binding polypeptide

KW - Light-harvesting complex II

KW - N-terminal sequence

KW - Photosystem II

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U2 - 10.1016/0014-5793(89)80020-1

DO - 10.1016/0014-5793(89)80020-1

M3 - Article

AN - SCOPUS:0342708743

SN - 0014-5793

VL - 249

SP - 79

EP - 82

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Detection of calcium binding by photosystem II polypeptides immobilised onto nitrocellulose membrane

Abstract

Keywords

ASJC Scopus subject areas

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