CO2-limitation-inducible Green Recovery of fatty acids from cyanobacterial biomass

Xinyao Liu, Sarah Fallon, Jie Sheng, Roy Curtiss

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71 Scopus citations


Using genetically modified cyanobacterial strains, we engineered a Green Recovery strategy to convert membrane lipids into fatty acids for economical and environmentally sustainable biofuel production. The Green Recovery strategy utilizes lipolytic enzymes under the control of promoters induced by CO 2 limitation. Data indicate that strains of the cyanobacterium Synechocystis sp. PCC6803 engineered for Green Recovery underwent degradation of membrane diacylglycerols upon CO2 limitation, leading to release of fatty acids into the culture medium. Recovered fatty acid yields of 36.1 x 10-12 mg/cell were measured in one of the engineered strains (SD239). Green Recovery can be incorporated into previously constructed fatty-acid-secretion strains, enabling fatty acid recovery from the remaining cyanobacterial biomass that will be generated during fatty acid biofuel production in photobioreactors.

Original languageEnglish (US)
Pages (from-to)6905-6908
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number17
StatePublished - Apr 26 2011

ASJC Scopus subject areas

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