Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses

Ruslan P. Kurta; Jeffrey J. Donatelli; Chun Hong Yoon; Peter Berntsen; Johan Bielecki; Benedikt J. Daurer; Hasan Demirci; Petra Fromme; Max Felix Hantke; Filipe R.N.C. Maia; Anna Munke; Carl Nettelblad; Kanupriya Pande; Hemanth K.N. Reddy; Jonas A. Sellberg; Raymond G. Sierra; Martin Svenda; Gijs Van Der Schot; Ivan A. Vartanyants; Garth J. Williams; P. Lourdu Xavier; Andrew Aquila; Peter H. Zwart; Adrian P. Mancuso

doi:10.1103/PhysRevLett.119.158102

Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses

Ruslan P. Kurta, Jeffrey J. Donatelli, Chun Hong Yoon, Peter Berntsen, Johan Bielecki, Benedikt J. Daurer, Hasan Demirci, Petra Fromme, Max Felix Hantke, Filipe R.N.C. Maia, Anna Munke, Carl Nettelblad, Kanupriya Pande, Hemanth K.N. Reddy, Jonas A. Sellberg, Raymond G. Sierra, Martin Svenda, Gijs Van Der Schot, Ivan A. Vartanyants, Garth J. WilliamsP. Lourdu Xavier, Andrew Aquila, Peter H. Zwart, Adrian P. Mancuso

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Abstract

We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our results anticipate exciting opportunities for XFEL studies of the structure and dynamics of nanoscale objects by means of angular correlations.

Original language	English (US)
Article number	158102
Journal	Physical Review Letters
Volume	119
Issue number	15
DOIs	https://doi.org/10.1103/PhysRevLett.119.158102
State	Published - Oct 12 2017

ASJC Scopus subject areas

Physics and Astronomy(all)

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10.1103/PhysRevLett.119.158102

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Kurta, R. P., Donatelli, J. J., Yoon, C. H., Berntsen, P., Bielecki, J., Daurer, B. J., Demirci, H., Fromme, P., Hantke, M. F., Maia, F. R. N. C., Munke, A., Nettelblad, C., Pande, K., Reddy, H. K. N., Sellberg, J. A., Sierra, R. G., Svenda, M., Van Der Schot, G., Vartanyants, I. A., ... Mancuso, A. P. (2017). Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses. Physical Review Letters, 119(15), Article 158102. https://doi.org/10.1103/PhysRevLett.119.158102

Kurta, RP, Donatelli, JJ, Yoon, CH, Berntsen, P, Bielecki, J, Daurer, BJ, Demirci, H, Fromme, P, Hantke, MF, Maia, FRNC, Munke, A, Nettelblad, C, Pande, K, Reddy, HKN, Sellberg, JA, Sierra, RG, Svenda, M, Van Der Schot, G, Vartanyants, IA, Williams, GJ, Xavier, PL, Aquila, A, Zwart, PH & Mancuso, AP 2017, 'Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses', Physical Review Letters, vol. 119, no. 15, 158102. https://doi.org/10.1103/PhysRevLett.119.158102

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title = "Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses",

abstract = "We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our results anticipate exciting opportunities for XFEL studies of the structure and dynamics of nanoscale objects by means of angular correlations.",

author = "Kurta, {Ruslan P.} and Donatelli, {Jeffrey J.} and Yoon, {Chun Hong} and Peter Berntsen and Johan Bielecki and Daurer, {Benedikt J.} and Hasan Demirci and Petra Fromme and Hantke, {Max Felix} and Maia, {Filipe R.N.C.} and Anna Munke and Carl Nettelblad and Kanupriya Pande and Reddy, {Hemanth K.N.} and Sellberg, {Jonas A.} and Sierra, {Raymond G.} and Martin Svenda and {Van Der Schot}, Gijs and Vartanyants, {Ivan A.} and Williams, {Garth J.} and Xavier, {P. Lourdu} and Andrew Aquila and Zwart, {Peter H.} and Mancuso, {Adrian P.}",

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doi = "10.1103/PhysRevLett.119.158102",

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AU - Kurta, Ruslan P.

AU - Donatelli, Jeffrey J.

AU - Yoon, Chun Hong

AU - Berntsen, Peter

AU - Bielecki, Johan

AU - Daurer, Benedikt J.

AU - Demirci, Hasan

AU - Fromme, Petra

AU - Hantke, Max Felix

AU - Maia, Filipe R.N.C.

AU - Munke, Anna

AU - Nettelblad, Carl

AU - Pande, Kanupriya

AU - Reddy, Hemanth K.N.

AU - Sellberg, Jonas A.

AU - Sierra, Raymond G.

AU - Svenda, Martin

AU - Van Der Schot, Gijs

AU - Vartanyants, Ivan A.

AU - Williams, Garth J.

AU - Xavier, P. Lourdu

AU - Aquila, Andrew

AU - Zwart, Peter H.

AU - Mancuso, Adrian P.

PY - 2017/10/12

Y1 - 2017/10/12

N2 - We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our results anticipate exciting opportunities for XFEL studies of the structure and dynamics of nanoscale objects by means of angular correlations.

AB - We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our results anticipate exciting opportunities for XFEL studies of the structure and dynamics of nanoscale objects by means of angular correlations.

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Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses

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