Construction and in vivo testing of prokaryotic riboregulators

Alexander Green

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations


RNAs that are transcribed and self-assemble within living cells are valuable tools for regulating and organizing cellular activities. Riboregulators, in particular, have been widely used for modulating translation and transcription in response to cognate transactivating or trigger RNAs, enabling cells to evaluate logic operations and to respond to environmental cues. Herein we detail a set of methods for the rapid construction and testing of prokaryotic riboregulators in Escherichia coli. These methods enable construction of dozens of riboregulator plasmids at the same time without the use of restriction enzymes. Furthermore, they facilitate rapid screening of devices and can be applied to a variety of other self-assembling in vivo RNA systems.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages18
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Cloning
  • Diagnostic
  • Plasmid
  • Regulation
  • Riboregulator
  • Riboswitch
  • Self-assembly
  • Sensor
  • Toehold switch
  • Transformation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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