Conformational transition in DNA on a cold surface

X. Z. Feng, R. Bash, P. Balagurumoorthy, D. Lohr, R. E. Harrington, Stuart Lindsay

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


The contour length of DNA fragments, deposited and imaged on mica under buffer, was measured as a function of deposition temperature. Extended DNA molecules (on Ni- and silane-treated surfaces) contract rapidly with falling temperature, approaching the contour length of A-DNA at 2°C. The contraction is not unique to a specific sequence and does not occur in solution at 2°C or on a surface at 25°C, indicating that it arises from a combination of low temperature and surface contact. It is probably a consequence of reduced water activity at a cold surface.

Original languageEnglish (US)
Pages (from-to)593-596
Number of pages4
JournalNucleic acids research
Issue number2
StatePublished - Jan 15 2000

ASJC Scopus subject areas

  • Genetics


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