Comparison of the domain-level organization of starch hydrolases and related enzymes

Structure-prediction and hydrophobic-cluster analysis of several starch hydrolases and related enzymes indicated the organization of eleven domain types. Most enzymes possess a catalytic (β/α)₈-barrel and a smaller C-terminal domain as seen in crystal structures of α-amylase and cyclodextrin glucanotransferase. Some also have a starch-granule-binding domain. Enzymes breaking or forming endo-α-1,6 linkages contain domains N-terminal to the (β/α)₈-barrel.