Coffee with High but Not Low Caffeine Content Augments Fluid and Electrolyte Excretion at Rest

Adam D. Seal, Costas N. Bardis, Anna Gavrieli, Petros Grigorakis, J. D. Adams, Giannis Arnaoutis, Mary Yannakoulia, Stavros A. Kavouras

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Background: Low levels of caffeine ingestion do not induce dehydration at rest, while it is not clear if larger doses do have an acute diuretic effect. The aim of the present investigation was to examine the acute effect of low and high levels of caffeine, via coffee, on fluid balance in habitual coffee drinkers (at least one per day) at rest. Methods: Ten healthy adults (eight males and two females; age: 27 ± 5 years, weight: 89.5 ± 14.8 kg, height: 1.75 ± 0.08 m, and body mass index: 29.1 ± 4.4 kg m−2) ingested 200 mL of water (W), coffee with low caffeine (3 mg kg−1, LCAF), or coffee with high caffeine (6 mg kg−1, HCAF) on three respective separate occasions. All sessions were performed at 09:00 in the morning in a counterbalanced, crossover manner, at least 5 days apart. Subjects remained in the laboratory while urine samples were collected every 60 min for 3 h post ingestion. Results: Absolute caffeine consumption was 269 ± 45 and 537 ± 89 mg for the LCAF and HCAF, respectively. Coffee ingestion at the HCAF trial induced greater diuresis during the 3-h period (613 ± 101 mL, P < 0.05), when compared to W (356 ± 53 mL) and LCAF (316 ± 38 mL). In addition, cumulative urinary osmotic excretion was significantly greater in the HCAF (425 ± 92 mmol, P < 0.05), as compared to the W (249 ± 36 mmol) and LCAF (177 ± 16 mmol) trials. Conclusion: The data indicate that caffeine intake of 6 mg kg−1 in the form of coffee can induce an acute diuretic effect, while 3 mg kg−1 do not disturb fluid balance in healthy casual coffee drinking adults at rest.

Original languageEnglish (US)
Article number40
JournalFrontiers in Nutrition
StatePublished - Aug 18 2017
Externally publishedYes


  • caffeine
  • coffee
  • dehydration
  • electrolytes
  • fluid balance
  • hydration
  • hypohydration

ASJC Scopus subject areas

  • Food Science
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics


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