TY - GEN
T1 - Chromophore-free tissue sealing and repair using mid infrared lasers
AU - Ridha, Inam
AU - Basiri, Ali
AU - Gudesala, Sudhakar
AU - Ghosh, Deepanjan
AU - Lee, Jung Keun
AU - Kilbourne, Jacquelyn
AU - Yao, Yu
AU - Rege, Kaushal
N1 - Publisher Copyright:
© 2019 Omnipress - All rights reserved.
PY - 2019
Y1 - 2019
N2 - Statement of Purpose: Sutures, staple, and conventional glues are commonly used to approximate tissue edges in surgery and wound healing. However, poor strength, infection, dehiscence, leakage, and / or acute inflammation are common complications associated with these methods. Laser-activated tissue sealing, in which, laser light energy is used to facilitate biomaterial incorporation with the tissue, provides an alternative approach for wound closure. Traditionally, light-absorbing chromophores and nanoparticles have been employed for converting near infrared (NIR) laser light to heat, resulting in the photothermal fusion of the sealant biomaterial with soft tissues. We now demonstrate a novel approach for sealing tissues without the need for chromophores using mid infrared (midIR) laser light. We characterized the absorption of midIR light by several different biomaterials and investigated the rise in local temperature at different laser powers. Optimal operating conditions were employed for midIR based photothermal sealing of incised / ruptured tissue ex vivo and using different skin surgical models in live mice. Recovery of mechanical properties including tensile strength and burst and leak pressures, in concert with histopathology analyses, were employed to determine the efficacy of the seal. The effect of midIR light on cell and tissue viability was also determined. Our results demonstrate that midIR lasers can be used for rapid sealing of soft tissues using conventional biomaterials without the need for chromophores or nanoparticles, which is a significant advantage for rapidly translating this technology in the clinic.
AB - Statement of Purpose: Sutures, staple, and conventional glues are commonly used to approximate tissue edges in surgery and wound healing. However, poor strength, infection, dehiscence, leakage, and / or acute inflammation are common complications associated with these methods. Laser-activated tissue sealing, in which, laser light energy is used to facilitate biomaterial incorporation with the tissue, provides an alternative approach for wound closure. Traditionally, light-absorbing chromophores and nanoparticles have been employed for converting near infrared (NIR) laser light to heat, resulting in the photothermal fusion of the sealant biomaterial with soft tissues. We now demonstrate a novel approach for sealing tissues without the need for chromophores using mid infrared (midIR) laser light. We characterized the absorption of midIR light by several different biomaterials and investigated the rise in local temperature at different laser powers. Optimal operating conditions were employed for midIR based photothermal sealing of incised / ruptured tissue ex vivo and using different skin surgical models in live mice. Recovery of mechanical properties including tensile strength and burst and leak pressures, in concert with histopathology analyses, were employed to determine the efficacy of the seal. The effect of midIR light on cell and tissue viability was also determined. Our results demonstrate that midIR lasers can be used for rapid sealing of soft tissues using conventional biomaterials without the need for chromophores or nanoparticles, which is a significant advantage for rapidly translating this technology in the clinic.
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M3 - Conference contribution
AN - SCOPUS:85065433434
T3 - Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
SP - 316
BT - Society for Biomaterials Annual Meeting and Exposition 2019
PB - Society for Biomaterials
T2 - 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
Y2 - 3 April 2019 through 6 April 2019
ER -