@article{009d14f6126e4ac1b0517553e3b948e1,
title = "Charge-Sensitive Optical Detection of Binding Kinetics between Phage-Displayed Peptide Ligands and Protein Targets",
abstract = "Phage display technology has been a powerful tool in peptide drug development. However, the supremacy of phage display-based peptide drug discovery is plagued by the follow-up process of peptides synthesis, which is costly and time consuming, but is necessary for the accurate measurement of binding kinetics in order to properly triage the best peptide leads during the affinity maturation stages. A sensitive technology is needed for directly measuring the binding kinetics of peptides on phages to reduce the time and cost of the entire process. Here, we show the capability of a charge-sensitive optical detection (CSOD) method for the direct quantification of binding kinetics of phage-displayed peptides to their target protein, using whole phages. We anticipate CSOD will contribute to streamline the process of phage display-based drug discovery.",
keywords = "binding kinetics, charge-sensitive optical detection, label-free, peptide, phage-display, protein",
author = "Runli Liang and Yingnan Zhang and Guangzhong Ma and Shaopeng Wang",
note = "Funding Information: µM [12,13] and the detection throughput can be further improved up to 48 binding pairs per microplate. We hope that these unique features of CSOD will make it a useful tool for www.mdpi.costudying thembinding/xxx/s1, Figuof PCSK9-bindingre S1: CSOD system noise anapeptides. lysis; Figure S2: Frequency dependency of the oscillation amplitude of a large diameter fiber; Figure S3: Comparison of CSOD signal long- term stability f5. Conclusionsor small and large diameter fibers. Source Data.xlsx is source data for Figures 2–4. We demonstrated the direct quantification of binding kinetics between phage-displayed peptides and the corresponding protein target by CSOD technology. We have shown that CSOD can be used to distinguish different binding kinetics of different peptides displayed obryigpinhaal gderadfti spprelapyartaetciohnn,o Rl.oLg.;y w. TrihtiengC—SOreDvi-emw eaansdu erdeditidngis,sYo.cZi.a, tGio.Mn.c, oannds tSa.nWts. Awlle areutvhaolrisd haatvede rbeyadt hanedl iategrraeetudrteo rthepe oprutbeldishveadl uveesrsmione aosfu threed mwaniuthscsryipnt.t hetic peptides. These results show that CSOD can be incorporated into a diversity display pipeline for rapidly triaging the Funding: This research was funded by National Institutes of Health grant number R33CA202834, best peptide leads during affinity maturation stages and can increase the productivity of for providinglibrary screening.PCSK9 protein. Funding Information: Funding: This research was funded by National Institutes of Health grant number R33CA202834, R44GM139535, and Genentech Inc. grant number CLL-016354. Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2022",
month = jun,
doi = "10.3390/bios12060394",
language = "English (US)",
volume = "12",
journal = "Biosensors",
issn = "2079-6374",
number = "6",
}