TY - GEN
T1 - Characterization of a compact and highly sensitive fluorescence-based detection system for point-of-care applications
AU - Obahiagbon, Uwadiae
AU - Kullman, Dixie
AU - Smith, Joseph T.
AU - Katchman, Benjamin A.
AU - Arafa, Hany
AU - Anderson, Karen
AU - Blain Christen, Jennifer
N1 - Funding Information:
This material is based on work supported by the NSF under Grant No. NSF/IIS-1521904 under the Smart and Connected Health program.
Publisher Copyright:
© 2016 IEEE.
PY - 2016/12/27
Y1 - 2016/12/27
N2 - This work details the design and characterization of a low-cost, portable and highly sensitive fluorescence detection system intended for use in a compact and disposable point-of-care (POC) device. The detection device leverages time integration to improve the signal to noise ratio (SNR) compared to instantaneous measurements. It also eliminates complicated focusing optics and electronics typically found in bulky and expensive laboratory-scale devices. Characterization was performed by measuring a series of logarithmically scaled dilutions of 1 μm Nile Red fluorescent microspheres immobilized on microscope slides. This approach eliminates assay dependencies and elucidates the actual system performance. A theoretical model that predicts the time-integrated output voltage profile of the sensor was developed; this prediction is useful for evaluating any lens-free fluorescent system based on a set of filters and choice of fluorophore. By relating the fluorophore concentration, system design parameters, and the output voltage, the model matches well with the empirical data and the limit of determination (LOD) for Nile Red is 20 particles. This system provides a sensitive and potentially low-cost device for fluorescent diagnosis in an integrated and compact/miniaturized POC device, lab-on-chip or a table-top reader.
AB - This work details the design and characterization of a low-cost, portable and highly sensitive fluorescence detection system intended for use in a compact and disposable point-of-care (POC) device. The detection device leverages time integration to improve the signal to noise ratio (SNR) compared to instantaneous measurements. It also eliminates complicated focusing optics and electronics typically found in bulky and expensive laboratory-scale devices. Characterization was performed by measuring a series of logarithmically scaled dilutions of 1 μm Nile Red fluorescent microspheres immobilized on microscope slides. This approach eliminates assay dependencies and elucidates the actual system performance. A theoretical model that predicts the time-integrated output voltage profile of the sensor was developed; this prediction is useful for evaluating any lens-free fluorescent system based on a set of filters and choice of fluorophore. By relating the fluorophore concentration, system design parameters, and the output voltage, the model matches well with the empirical data and the limit of determination (LOD) for Nile Red is 20 particles. This system provides a sensitive and potentially low-cost device for fluorescent diagnosis in an integrated and compact/miniaturized POC device, lab-on-chip or a table-top reader.
UR - http://www.scopus.com/inward/record.url?scp=85010702921&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85010702921&partnerID=8YFLogxK
U2 - 10.1109/HIC.2016.7797711
DO - 10.1109/HIC.2016.7797711
M3 - Conference contribution
AN - SCOPUS:85010702921
T3 - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
SP - 117
EP - 120
BT - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
Y2 - 9 November 2016 through 11 November 2016
ER -