TY - GEN
T1 - Centrifugal automation of a highly customizable rapid-elisa test towards detection of cervical cancer in point-of-care settings
AU - Mishra, R.
AU - Alam, R.
AU - McAuley, D.
AU - Bharaj, T.
AU - Kinahan, D.
AU - Nwankire, C.
AU - Anderson, Karen
AU - Ducrée, J.
N1 - Funding Information:
This study was supported by a collaborative institutional grant from Dublin City University and Arizona State University.
PY - 2016
Y1 - 2016
N2 - Infections of high-risk strains of human papilloma virus (HPV16-E7) have been directly linked with cervical carcinogenesis [1]. Despite pervasive HPV testing and vaccinations, resource-poor regions continue to be affected. We present here a fully automated, bead-based Rapid Antigenic Protein In-situ Display (RAPID) Enzyme Linked Immunosorbent Assay (ELISA) for the detection of the onco-proteins of HPV16 E7 [1]. Advanced centrifugal flow control is implemented by a novel router based on lipophilic membranes in an event-trigger valving architecture [2]. By simply replacing the charge of RAPID-functionalized beads in the incubation chamber, the system may be customized for the detection of other biomarkers.
AB - Infections of high-risk strains of human papilloma virus (HPV16-E7) have been directly linked with cervical carcinogenesis [1]. Despite pervasive HPV testing and vaccinations, resource-poor regions continue to be affected. We present here a fully automated, bead-based Rapid Antigenic Protein In-situ Display (RAPID) Enzyme Linked Immunosorbent Assay (ELISA) for the detection of the onco-proteins of HPV16 E7 [1]. Advanced centrifugal flow control is implemented by a novel router based on lipophilic membranes in an event-trigger valving architecture [2]. By simply replacing the charge of RAPID-functionalized beads in the incubation chamber, the system may be customized for the detection of other biomarkers.
KW - Centrifugal microfluidics
KW - Cervical cancer
KW - Lab-on-a-Disc (LoaD)
KW - Lipophilic membranes
KW - RAPID ELISA
KW - Routing
UR - http://www.scopus.com/inward/record.url?scp=85014273726&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014273726&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85014273726
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 844
EP - 845
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Y2 - 9 October 2016 through 13 October 2016
ER -