Binding event measurement using a chip calorimeter coupled to magnetic beads

L. M. Ahmad, B. Towe, A. Wolf, F. Mertens, J. Lerchner

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Chip calorimetry is a promising method for the label-free detection of biomolecules. Miniaturized calorimetric devices using stagnant droplets require sample volumes in the nano-liter range, but exhibit some limitations regarding volume specific signal resolution, baseline stability, and evaporation. In this paper, we describe the application of a silicon chip based flow-through calorimeter for the detection of biotin and aptamers. To achieve high sensitivity, receptors coupled to magnetic beads were used. Streptavidin served as a receptor for the detection of biotin. For the first time, the chip-calorimetric response to DNA hybridization has been successfully demonstrated. For these experiments, the biotinylated complement of the DNA oligomer was coupled to the streptavidin surface of magnetic beads.

Original languageEnglish (US)
Pages (from-to)239-245
Number of pages7
JournalSensors and Actuators, B: Chemical
Issue number1
StatePublished - Mar 4 2010


  • Biosensor
  • Biotin-streptavidin reaction
  • Chip-calorimetry
  • DNA hybridization

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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