Antioxidant effect of human selenium-containing single-chain Fv in rat cardiac myocytes

Rui Huo; Yi Shi; Jun Jie Xu; Fei Yan; Shao Wu Lü; Jia Ming Su; Yu Jing Duan; Jia Fan; Bo Ning; Deng Li Cong; Gang Lin Yan; Gui Min Luo; Jing Yan Wei

Antioxidant effect of human selenium-containing single-chain Fv in rat cardiac myocytes

Rui Huo
, Yi Shi
, Jun Jie Xu
, Fei Yan
, Shao Wu Lü
, Jia Ming Su
, Yu Jing Duan
, Jia Fan
, Bo Ning
, Deng Li Cong
, Gang Lin Yan
, Gui Min Luo
, Jing Yan Wei

Research output: Contribution to journal › Article › peer-review

Abstract

Reactive oxygen species(ROS) plays a key role in human heart diseases. Glutathione peroxidase(GPX) functions as an antioxidant as it catalyzes the reduction of hydroperoxide. In order to investigate the antioxidant effect of human selenium-containing single-chain Fv(Se-scFv-B3), a new mimic of GPX, a model system of hydrogen peroxide(H₂O₂)-induced rat cardiac myocyte damage was established. The cardiac myocyte damage was characterized in terms of cell viability, lipid peroxidation, cell membrane integrity, and intracellular H₂O₂ level. The Se-scFv-B3 significantly reduced H₂O₂-induced cell damage as shown by the increase of cell viability, the decline of malondialdehyde(MDA) production, lactate dehydrogenase(LDH) release, and intracellular H₂O₂ level. So Se-scFv- B3 may have a great potential in the treatment of human heart diseases induced by ROS.

Original language	English (US)
Pages (from-to)	216-219
Number of pages	4
Journal	Chemical Research in Chinese Universities
Volume	25
Issue number	2
State	Published - 2009
Externally published	Yes

Keywords

Cardiac myocyte
Glutathione peroxidase
HO
Human single-chain Fv
Selenium

ASJC Scopus subject areas

General Chemistry

Cite this

@article{9332b053a1534a25a77d3b44caea6676,

title = "Antioxidant effect of human selenium-containing single-chain Fv in rat cardiac myocytes",

abstract = "Reactive oxygen species(ROS) plays a key role in human heart diseases. Glutathione peroxidase(GPX) functions as an antioxidant as it catalyzes the reduction of hydroperoxide. In order to investigate the antioxidant effect of human selenium-containing single-chain Fv(Se-scFv-B3), a new mimic of GPX, a model system of hydrogen peroxide(H2O2)-induced rat cardiac myocyte damage was established. The cardiac myocyte damage was characterized in terms of cell viability, lipid peroxidation, cell membrane integrity, and intracellular H2O2 level. The Se-scFv-B3 significantly reduced H2O2-induced cell damage as shown by the increase of cell viability, the decline of malondialdehyde(MDA) production, lactate dehydrogenase(LDH) release, and intracellular H2O2 level. So Se-scFv- B3 may have a great potential in the treatment of human heart diseases induced by ROS.",

keywords = "Cardiac myocyte, Glutathione peroxidase, HO, Human single-chain Fv, Selenium",

author = "Rui Huo and Yi Shi and Xu, \{Jun Jie\} and Fei Yan and L{\"u}, \{Shao Wu\} and Su, \{Jia Ming\} and Duan, \{Yu Jing\} and Jia Fan and Bo Ning and Cong, \{Deng Li\} and Yan, \{Gang Lin\} and Luo, \{Gui Min\} and Wei, \{Jing Yan\}",

year = "2009",

language = "English (US)",

volume = "25",

pages = "216--219",

journal = "Chemical Research in Chinese Universities",

issn = "1005-9040",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "2",

}

TY - JOUR

T1 - Antioxidant effect of human selenium-containing single-chain Fv in rat cardiac myocytes

AU - Huo, Rui

AU - Shi, Yi

AU - Xu, Jun Jie

AU - Yan, Fei

AU - Lü, Shao Wu

AU - Su, Jia Ming

AU - Duan, Yu Jing

AU - Fan, Jia

AU - Ning, Bo

AU - Cong, Deng Li

AU - Yan, Gang Lin

AU - Luo, Gui Min

AU - Wei, Jing Yan

PY - 2009

Y1 - 2009

N2 - Reactive oxygen species(ROS) plays a key role in human heart diseases. Glutathione peroxidase(GPX) functions as an antioxidant as it catalyzes the reduction of hydroperoxide. In order to investigate the antioxidant effect of human selenium-containing single-chain Fv(Se-scFv-B3), a new mimic of GPX, a model system of hydrogen peroxide(H2O2)-induced rat cardiac myocyte damage was established. The cardiac myocyte damage was characterized in terms of cell viability, lipid peroxidation, cell membrane integrity, and intracellular H2O2 level. The Se-scFv-B3 significantly reduced H2O2-induced cell damage as shown by the increase of cell viability, the decline of malondialdehyde(MDA) production, lactate dehydrogenase(LDH) release, and intracellular H2O2 level. So Se-scFv- B3 may have a great potential in the treatment of human heart diseases induced by ROS.

AB - Reactive oxygen species(ROS) plays a key role in human heart diseases. Glutathione peroxidase(GPX) functions as an antioxidant as it catalyzes the reduction of hydroperoxide. In order to investigate the antioxidant effect of human selenium-containing single-chain Fv(Se-scFv-B3), a new mimic of GPX, a model system of hydrogen peroxide(H2O2)-induced rat cardiac myocyte damage was established. The cardiac myocyte damage was characterized in terms of cell viability, lipid peroxidation, cell membrane integrity, and intracellular H2O2 level. The Se-scFv-B3 significantly reduced H2O2-induced cell damage as shown by the increase of cell viability, the decline of malondialdehyde(MDA) production, lactate dehydrogenase(LDH) release, and intracellular H2O2 level. So Se-scFv- B3 may have a great potential in the treatment of human heart diseases induced by ROS.

KW - Cardiac myocyte

KW - Glutathione peroxidase

KW - HO

KW - Human single-chain Fv

KW - Selenium

UR - https://www.scopus.com/pages/publications/80051813454

UR - https://www.scopus.com/pages/publications/80051813454#tab=citedBy

M3 - Article

AN - SCOPUS:80051813454

SN - 1005-9040

VL - 25

SP - 216

EP - 219

JO - Chemical Research in Chinese Universities

JF - Chemical Research in Chinese Universities

IS - 2

ER -

Antioxidant effect of human selenium-containing single-chain Fv in rat cardiac myocytes

Abstract

Keywords

ASJC Scopus subject areas

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