An unbiased genome-wide view of the mutation rate and spectrum of the endosymbiotic bacterium teredinibacter turnerae

Mutations contribute to genetic variation in all living systems. Thus, precise estimates of mutation rates and spectra across a diversity of organisms are required for a full comprehension of evolution. Here, a mutation-accumulation (MA) assay was carried out on the endosymbiotic bacterium Teredinibacter turnerae. After 3,025 generations, base-pair substitutions (BPSs) and insertion–deletion (indel) events were characterized by whole-genome sequencing analysis of 47 independent MA lines, yielding a BPS rate of 1.14 10⁹ per site per generation and indel rate of 1.55 10¹⁰ events per site per generation, which are among the highest within free-living and facultative intracellular bacteria. As in other endosymbionts, a significant bias of BPSs toward A/T and an excess of deletion mutations over insertion mutations are observed for these MA lines. However, even with a deletion bias, the genome remains relatively large (5.2 Mb) for an endosymbiotic bacterium. The estimate of the effective population size (N_e) in T. turnerae is quite high and comparable to free-living bacteria (4.5 10⁷), suggesting that the heavy bottlenecking associated with many endosymbiotic relationships is not prevalent during the life of this endosymbiont. The efficiency of selection scales with increasing N_e and such strong selection may have been operating against the deletion bias, preventing genome erosion.