An ultra-rapid cryo-technique for complex organisms

T. Irdani, Angelo Fortunato, R. Torre

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


The soil nematode Caenorhabditis elegans is an excellent research model in cell biology, human disease and developmental studies. In this study, a novel cryopreservation technique based on a rapid cooling procedure, previously established for juveniles, was applied to adult-worms. Here we demonstrated that adults of C. elegans, a complex metazoan organism, survive to a rapid cooling and storage in liquid nitrogen (-196 °C) with a very high survival percentage (85%). The procedure relies on a Low CryoProtectant Technique (LCPT) and Ultra Rapid Cooling (URC). The high cooling rate is achieved through the reduction of sample volumes and the effectiveness of a nylon carrier. Our technique complies with the requirements for vitrification to occur. The main distinctive characters of this cryopreservation technique compared to other methods, e.g. Slow Freezing and Vitrification, are presented. Our results show that this cryopreservation method is valid for both unicellular and multicellular organisms; it is suitable for short or long time storage in liquid-nitrogen. This technique promises to be a unique and simpler method for cryostorage of cells, organisms and tissues.

Original languageEnglish (US)
Pages (from-to)391-397
Number of pages7
Issue number3
StatePublished - Dec 1 2015


  • C. elegans
  • Cooling-rate
  • Cryopreservation
  • Long-term storage
  • Low cryoprotectant technique
  • Organisms cryostorage
  • Thermal-analysis
  • Vitrification

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences


Dive into the research topics of 'An ultra-rapid cryo-technique for complex organisms'. Together they form a unique fingerprint.

Cite this