An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins

Oliver P. Windram; Brandon Weber; Mohamed A. Jaffer; Edward P. Rybicki; Dionne N. Shepherd; Arvind Varsani

doi:10.1007/s00705-007-0025-2

An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins

Oliver P. Windram, Brandon Weber, Mohamed A. Jaffer, Edward P. Rybicki, Dionne N. Shepherd, Arvind Varsani

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Development of vaccine strategies against human papillomavirus (HPV), which causes cervical cancer, is a priority. We investigated the use of virus-like particles (VLPs) of the most prevalent type, HPV-16, as carriers of foreign proteins. Green fluorescent protein (GFP) was fused to the N or C terminus of both L1 and L2, with L2 chimeras being co-expressed with native L1. Purified chimaeric VLPs were comparable in size (∼55 nm) to native HPV VLPs. Conformation-specific monoclonal antibodies (Mabs) bound to the VLPs, thereby indicating that they possibly retain their antigenicity. In addition, all of the VLPs encapsidated DNA in the range of 6-8 kb.

Original language	English (US)
Pages (from-to)	585-589
Number of pages	5
Journal	Archives of virology
Volume	153
Issue number	3
DOIs	https://doi.org/10.1007/s00705-007-0025-2
State	Published - Mar 2008
Externally published	Yes

ASJC Scopus subject areas

Virology

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10.1007/s00705-007-0025-2

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Windram, O. P., Weber, B., Jaffer, M. A., Rybicki, E. P., Shepherd, D. N., & Varsani, A. (2008). An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins. Archives of virology, 153(3), 585-589. https://doi.org/10.1007/s00705-007-0025-2

An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins. / Windram, Oliver P.; Weber, Brandon; Jaffer, Mohamed A. et al.
In: Archives of virology, Vol. 153, No. 3, 03.2008, p. 585-589.

Research output: Contribution to journal › Article › peer-review

Windram, OP, Weber, B, Jaffer, MA, Rybicki, EP, Shepherd, DN & Varsani, A 2008, 'An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins', Archives of virology, vol. 153, no. 3, pp. 585-589. https://doi.org/10.1007/s00705-007-0025-2

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title = "An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins",

abstract = "Development of vaccine strategies against human papillomavirus (HPV), which causes cervical cancer, is a priority. We investigated the use of virus-like particles (VLPs) of the most prevalent type, HPV-16, as carriers of foreign proteins. Green fluorescent protein (GFP) was fused to the N or C terminus of both L1 and L2, with L2 chimeras being co-expressed with native L1. Purified chimaeric VLPs were comparable in size (∼55 nm) to native HPV VLPs. Conformation-specific monoclonal antibodies (Mabs) bound to the VLPs, thereby indicating that they possibly retain their antigenicity. In addition, all of the VLPs encapsidated DNA in the range of 6-8 kb.",

author = "Windram, {Oliver P.} and Brandon Weber and Jaffer, {Mohamed A.} and Rybicki, {Edward P.} and Shepherd, {Dionne N.} and Arvind Varsani",

note = "Funding Information: We would like thank to Dr. Neil Christensen for providing the monoclonal antibodies used in this study. This research was funded by the Poliomyelitis Research Foundation of South Africa (grant #04/25) and the University of Cape Town start up grant. AV is supported by the Carnegie Corporation of New York.",

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AB - Development of vaccine strategies against human papillomavirus (HPV), which causes cervical cancer, is a priority. We investigated the use of virus-like particles (VLPs) of the most prevalent type, HPV-16, as carriers of foreign proteins. Green fluorescent protein (GFP) was fused to the N or C terminus of both L1 and L2, with L2 chimeras being co-expressed with native L1. Purified chimaeric VLPs were comparable in size (∼55 nm) to native HPV VLPs. Conformation-specific monoclonal antibodies (Mabs) bound to the VLPs, thereby indicating that they possibly retain their antigenicity. In addition, all of the VLPs encapsidated DNA in the range of 6-8 kb.

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An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins

Abstract

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