@inproceedings{663e825ade534e678b9a1f5a4f1a3d3e,
title = "Amyloid β analysis from microdissected brain cells using microfluidics and maldi mass spectrometry",
abstract = "Soluble amyloid β (Aβ) oligomers have been identified as the main species responsible for neuronal dysfunction in Alzheimer's Disease (AD), but details on their chemical nature are still not unraveled. We are developing a microfluidic assay to analyze Aβ species from microdissected human brain cells with MALDI mass spectrometry (MS) for chemical identification. Here, we report the capture of microdissected brain cells into microfluidic platforms, Aβ immunocapture with suitable antibodies, and Aβ42 species characterization using MALDI-MS and atomic force microscopy. This approach will allow to investigate soluble Aβ species in AD post-mortem brains providing insights into their role in AD.",
keywords = "Amyloid-beta, Laser-microdissection, Mass Spectrometry, Microfluidics",
author = "Villarreal, \{Jorvani Cruz\} and Ana Egatz-Gomez and Jiawei Liu and Robert Ros and Coleman, \{Paul D.\} and Alexandra Ros",
note = "Funding Information: We thank Dr. S. Lindsay for the support with the AFM instrument and CONACYT for financial support. Publisher Copyright: {\textcopyright} 2020 CBMS-0001; 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 ; Conference date: 04-10-2020 Through 09-10-2020",
year = "2020",
language = "English (US)",
series = "MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "711--712",
booktitle = "MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
}