TY - GEN
T1 - Amyloid β analysis from microdissected brain cells using microfluidics and maldi mass spectrometry
AU - Villarreal, Jorvani Cruz
AU - Egatz-Gomez, Ana
AU - Liu, Jiawei
AU - Ros, Robert
AU - Coleman, Paul D.
AU - Ros, Alexandra
N1 - Funding Information:
We thank Dr. S. Lindsay for the support with the AFM instrument and CONACYT for financial support.
Publisher Copyright:
© 2020 CBMS-0001
PY - 2020
Y1 - 2020
N2 - Soluble amyloid β (Aβ) oligomers have been identified as the main species responsible for neuronal dysfunction in Alzheimer's Disease (AD), but details on their chemical nature are still not unraveled. We are developing a microfluidic assay to analyze Aβ species from microdissected human brain cells with MALDI mass spectrometry (MS) for chemical identification. Here, we report the capture of microdissected brain cells into microfluidic platforms, Aβ immunocapture with suitable antibodies, and Aβ42 species characterization using MALDI-MS and atomic force microscopy. This approach will allow to investigate soluble Aβ species in AD post-mortem brains providing insights into their role in AD.
AB - Soluble amyloid β (Aβ) oligomers have been identified as the main species responsible for neuronal dysfunction in Alzheimer's Disease (AD), but details on their chemical nature are still not unraveled. We are developing a microfluidic assay to analyze Aβ species from microdissected human brain cells with MALDI mass spectrometry (MS) for chemical identification. Here, we report the capture of microdissected brain cells into microfluidic platforms, Aβ immunocapture with suitable antibodies, and Aβ42 species characterization using MALDI-MS and atomic force microscopy. This approach will allow to investigate soluble Aβ species in AD post-mortem brains providing insights into their role in AD.
KW - Amyloid-beta
KW - Laser-microdissection
KW - Mass Spectrometry
KW - Microfluidics
UR - http://www.scopus.com/inward/record.url?scp=85098254378&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85098254378&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85098254378
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 711
EP - 712
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -