A living cell array (LCA) for multiparameter cell metabolism studies

Sarah C. McQuaide; Mark R. Holl; Lloyd Burgess; Timothy Molter; Joseph Dragavon; A. Cody Young; Timothy Strovas; Judith Anderson; Alex Jen; Brandon Karlsgodt; Mary Lidstrom; Deirdre Meldrum

doi:10.1109/BIOROB.2006.1639217

A living cell array (LCA) for multiparameter cell metabolism studies

Sarah C. McQuaide, Mark R. Holl, Lloyd Burgess, Timothy Molter, Joseph Dragavon, A. Cody Young, Timothy Strovas, Judith Anderson, Alex Jen, Brandon Karlsgodt, Mary Lidstrom, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

6 Scopus citations

Abstract

A proof-of-platform-concept architecture for the analysis of living cell arrays is presented. Initial experiments conducted show a conceptual design for single-cell multiparameter measurements in real time. As a first parameter, extensive development and experimentation have been conducted on a sensor that will measure single-cell oxygen consumption. Wafer-level processing for chip fabrication and sensor deposition have been achieved, making possible the future goal of an automated system using disposable chips. The oxygen sensor has been tested for long-term adherence to a substrate in aqueous environments, biocompatibility with cell types of interest, and sensitivity to small changes in local oxygen concentration. A test device was made to verify the use of a gold foil barrier as an oxygen seal and shows good isolation of sealed areas.

Original language	English (US)
Title of host publication	Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006
Pages	971-976
Number of pages	6
DOIs	https://doi.org/10.1109/BIOROB.2006.1639217
State	Published - 2006
Externally published	Yes
Event	1st IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006 - Pisa, Italy Duration: Feb 20 2006 → Feb 22 2006

Publication series

Name	Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006
Volume	2006

Other

Other	1st IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006
Country/Territory	Italy
City	Pisa
Period	2/20/06 → 2/22/06

Keywords

Microfabrication
Oxygen barrier
Oxygen consumption
Platinum porphyrin
Single-cell

ASJC Scopus subject areas

Engineering(all)

Access to Document

10.1109/BIOROB.2006.1639217

Cite this

McQuaide, S. C., Holl, M. R., Burgess, L., Molter, T., Dragavon, J., Young, A. C., Strovas, T., Anderson, J., Jen, A., Karlsgodt, B., Lidstrom, M., & Meldrum, D. (2006). A living cell array (LCA) for multiparameter cell metabolism studies. In Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006 (pp. 971-976). Article 1639217 (Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006; Vol. 2006). https://doi.org/10.1109/BIOROB.2006.1639217

A living cell array (LCA) for multiparameter cell metabolism studies. / McQuaide, Sarah C.; Holl, Mark R.; Burgess, Lloyd et al.
Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006. 2006. p. 971-976 1639217 (Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006; Vol. 2006).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

McQuaide, SC, Holl, MR, Burgess, L, Molter, T, Dragavon, J, Young, AC, Strovas, T, Anderson, J, Jen, A, Karlsgodt, B, Lidstrom, M & Meldrum, D 2006, A living cell array (LCA) for multiparameter cell metabolism studies. in Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006., 1639217, Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006, vol. 2006, pp. 971-976, 1st IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006, Pisa, Italy, 2/20/06. https://doi.org/10.1109/BIOROB.2006.1639217

McQuaide SC, Holl MR, Burgess L, Molter T, Dragavon J, Young AC et al. A living cell array (LCA) for multiparameter cell metabolism studies. In Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006. 2006. p. 971-976. 1639217. (Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006). doi: 10.1109/BIOROB.2006.1639217

McQuaide, Sarah C. ; Holl, Mark R. ; Burgess, Lloyd et al. / A living cell array (LCA) for multiparameter cell metabolism studies. Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006. 2006. pp. 971-976 (Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006).

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abstract = "A proof-of-platform-concept architecture for the analysis of living cell arrays is presented. Initial experiments conducted show a conceptual design for single-cell multiparameter measurements in real time. As a first parameter, extensive development and experimentation have been conducted on a sensor that will measure single-cell oxygen consumption. Wafer-level processing for chip fabrication and sensor deposition have been achieved, making possible the future goal of an automated system using disposable chips. The oxygen sensor has been tested for long-term adherence to a substrate in aqueous environments, biocompatibility with cell types of interest, and sensitivity to small changes in local oxygen concentration. A test device was made to verify the use of a gold foil barrier as an oxygen seal and shows good isolation of sealed areas.",

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AU - Young, A. Cody

AU - Strovas, Timothy

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A living cell array (LCA) for multiparameter cell metabolism studies

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Keywords

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