TY - JOUR
T1 - A compact, low-cost, quantitative and multiplexed fluorescence detection platform for point-of-care applications
AU - Obahiagbon, Uwadiae
AU - Smith, Joseph T.
AU - Zhu, Meilin
AU - Katchman, Benjamin A.
AU - Arafa, Hany
AU - Anderson, Karen
AU - Blain Christen, Jennifer
N1 - Publisher Copyright:
© 2018 The Authors
PY - 2018/10/15
Y1 - 2018/10/15
N2 - An effective method of combating infectious diseases is the deployment of hand-held devices at the point-of-care (POC) for screening or self-monitoring applications. There is a need for very sensitive, low-cost and quantitative diagnostic devices. In this study, we present a low-cost, multiplexed fluorescence detection platform that has a high sensitivity and wide dynamic range. Our system features inexpensive 3 × 3 mm interference filters with a high stopband rejection, sharp transition edges, and greater than 90% transmission in the passband. In addition to the filters, we improve signal-to-noise ratio by leveraging time for accuracy using a charge-integration-based readout. The fluorescence sensing platform provides a sensitivity to photon flux of ∼1×104photons/mm2sec and has the potential for 2–3 orders of magnitude improvement in sensitivity over standard colorimetric detection that uses colored latex microspheres. We also detail the design, development, and characterization of our low-cost fluorescence detection platform and demonstrate 100% and 97.96% reduction in crosstalk probability and filter cost, respectively. This is achieved by reducing filter dimensions and ensuring appropriate channel isolation in a 2 × 2 array configuration. Practical considerations with low-cost interference filter system design, analysis, and system performance are also discussed. The performance of our platform is compared to that of a standard laboratory array scanner. We also demonstrate the detection of antibodies to human papillomavirus (HPV16) E7 protein, as a potential biomarker for early cervical cancer detection in human plasma.
AB - An effective method of combating infectious diseases is the deployment of hand-held devices at the point-of-care (POC) for screening or self-monitoring applications. There is a need for very sensitive, low-cost and quantitative diagnostic devices. In this study, we present a low-cost, multiplexed fluorescence detection platform that has a high sensitivity and wide dynamic range. Our system features inexpensive 3 × 3 mm interference filters with a high stopband rejection, sharp transition edges, and greater than 90% transmission in the passband. In addition to the filters, we improve signal-to-noise ratio by leveraging time for accuracy using a charge-integration-based readout. The fluorescence sensing platform provides a sensitivity to photon flux of ∼1×104photons/mm2sec and has the potential for 2–3 orders of magnitude improvement in sensitivity over standard colorimetric detection that uses colored latex microspheres. We also detail the design, development, and characterization of our low-cost fluorescence detection platform and demonstrate 100% and 97.96% reduction in crosstalk probability and filter cost, respectively. This is achieved by reducing filter dimensions and ensuring appropriate channel isolation in a 2 × 2 array configuration. Practical considerations with low-cost interference filter system design, analysis, and system performance are also discussed. The performance of our platform is compared to that of a standard laboratory array scanner. We also demonstrate the detection of antibodies to human papillomavirus (HPV16) E7 protein, as a potential biomarker for early cervical cancer detection in human plasma.
KW - Colorimetry
KW - Diagnostics
KW - Fluorescence
KW - Limit of detection
KW - Multiplexed
KW - Point-of-care
UR - http://www.scopus.com/inward/record.url?scp=85048179187&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048179187&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2018.04.002
DO - 10.1016/j.bios.2018.04.002
M3 - Article
C2 - 29894852
AN - SCOPUS:85048179187
SN - 0956-5663
VL - 117
SP - 153
EP - 160
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -