Additional file 5: of The association of genomic lesions and PD-1/PD-L1 expression in resected triple-negative breast cancers

Figure S5. FISH validation of 9p24.1 amplicon. A) DNA content histogram of flow-sorted TNBC-29. B) Chromosome 9 Comparative Genomic Hybridization plot with (log2 ratio >1) gain of JAK2 locus (arrow) at 9p24.1. C) Multi-color FISH assay [5′JAK2[9p24](green)/ 3′JAK2[9p24](red)/CEN 9(aqua)] image indicates 3–5 intact JAK2 signals and 2–3 CEN 9 signals. Abbreviations: FISH fluorescence in situ hybridization, TNBC triple-negative breast cancer. (PPTX 776 kb)