4ZWJ : Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser

  • Yanyong Kang (Contributor)
  • X. Edward Zhou (Contributor)
  • Xiang Gao (Contributor)
  • Yuanzheng He (Contributor)
  • Wei Liu (Contributor)
  • Andrii Ishchenko (Contributor)
  • Anton Barty (Contributor)
  • Thomas A. White (Contributor)
  • Oleksandr M. Yefanov (Contributor)
  • Gye Won Han (Contributor)
  • Qingping Xu (Contributor)
  • Parker W. De Waal (Contributor)
  • Jiyuan Ke (Contributor)
  • M. H.Eileen Tan (Contributor)
  • Chenghai Zhang (Contributor)
  • Arne Moeller (Contributor)
  • Graham M. West (Contributor)
  • Bruce D. Pascal (Contributor)
  • Ned Van Eps (Contributor)
  • Lydia N. Caro (Contributor)
  • Sergey A. Vishnivetskiy (Contributor)
  • Regina J. Lee (Contributor)
  • Kelly M. Suino-Powell (Contributor)
  • Xin Gu (Contributor)
  • Kuntal Pal (Contributor)
  • Jinming Ma (Contributor)
  • Xiaoyong Zhi (Contributor)
  • Sébastien Boutet (Contributor)
  • Garth J. Williams (Contributor)
  • Marc Messerschmidt (Contributor)
  • Cornelius Gati (Contributor)
  • Nadia A. Zatsepin (Contributor)
  • Dingjie Wang (Contributor)
  • Daniel James (Contributor)
  • Shibom Basu (Contributor)
  • Shatabdi Roy-Chowdhury (Contributor)
  • Chelsie E. Conrad (Contributor)
  • Jesse Coe (Contributor)
  • Haiguang Liu (Contributor)
  • Stella Lisova (Contributor)
  • Christopher Kupitz (Contributor)
  • Ingo Grotjohann (Contributor)
  • Raimund Fromme (Contributor)
  • Yi Jiang (Contributor)
  • Minjia Tan (Contributor)
  • Huaiyu Yang (Contributor)
  • Jun Li (Contributor)
  • Meitian Wang (Contributor)
  • Zhong Zheng (Contributor)
  • Dianfan Li (Contributor)
  • Nicole Howe (Contributor)
  • Yingming Zhao (Contributor)
  • Jörg Standfuss (Contributor)
  • Kay Diederichs (Contributor)
  • Yuhui Dong (Contributor)
  • Clinton S. Potter (Contributor)
  • Bridget Carragher (Contributor)
  • Martin Caffrey (Contributor)
  • Hualiang Jiang (Contributor)
  • Henry N. Chapman (Contributor)
  • John C. H. Spence (Contributor)
  • Petra Fromme (Contributor)
  • Uwe Weierstall (Contributor)
  • Oliver P. Ernst (Contributor)
  • Vsevolod Katritch (Contributor)
  • Vsevolod V. Gurevich (Contributor)
  • Patrick R. Griffin (Contributor)
  • Wayne L. Hubbell (Contributor)
  • Raymond C. Stevens (Contributor)
  • Vadim Cherezov (Contributor)
  • Karsten Melcher (Contributor)
  • H. Eric Xu (Contributor)



Experimental Technique/Method:X-RAY DIFFRACTION
Release Date:2015-07-29
Deposition Date:2015-05-19
Revision Date:2015-08-05#2015-08-12#2018-02-14
Molecular Weight:403444.41
Macromolecule Type:Protein
Residue Count:3624
Atom Site Count:24665

G-protein-coupled receptors (GPCRs) signal primarily through G proteins or arrestins. Arrestin binding to GPCRs blocks G protein interaction and redirects signalling to numerous G-protein-independent pathways. Here we report the crystal structure of a constitutively active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin, determined by serial femtosecond X-ray laser crystallography. Together with extensive biochemical and mutagenesis data, the structure reveals an overall architecture of the rhodopsin-arrestin assembly in which rhodopsin uses distinct structural elements, including transmembrane helix 7 and helix 8, to recruit arrestin. Correspondingly, arrestin adopts the pre-activated conformation, with a ∼20° rotation between the amino and carboxy domains, which opens up a cleft in arrestin to accommodate a short helix formed by the second intracellular loop of rhodopsin. This structure provides a basis for understanding GPCR-mediated arrestin-biased signalling and demonstrates the power of X-ray lasers for advancing the frontiers of structural biology.
Date made available2015

Cite this