Description
Experimental Technique/Method:X-RAY DIFFRACTION
Resolution:2.9
Classification:MEMBRANE PROTEIN
Release Date:2015-04-22
Deposition Date:2015-02-18
Revision Date:2015-05-06#2015-05-13#2015-05-27#2017-09-06#2017-11-22#2018-02-14
Molecular Weight:47679.23
Macromolecule Type:Protein
Residue Count:414
Atom Site Count:3110
DOI:10.2210/pdb4yay/pdb
Abstract:
Angiotensin II type 1 receptor (AT(1)R) is a G protein-coupled receptor that serves as a primary regulator for blood pressure maintenance. Although several anti-hypertensive drugs have been developed as AT(1)R blockers (ARBs), the structural basis for AT(1)R ligand-binding and regulation has remained elusive, mostly due to the difficulties of growing high-quality crystals for structure determination using synchrotron radiation. By applying the recently developed method of serial femtosecond crystallography at an X-ray free-electron laser, we successfully determined the room-temperature crystal structure of the human AT(1)R in complex with its selective antagonist ZD7155 at 2.9-Å resolution. The AT(1)R-ZD7155 complex structure revealed key structural features of AT(1)R and critical interactions for ZD7155 binding. Docking simulations of the clinically used ARBs into the AT(1)R structure further elucidated both the common and distinct binding modes for these anti-hypertensive drugs. Our results thereby provide fundamental insights into AT(1)R structure-function relationship and structure-based drug design.
Resolution:2.9
Classification:MEMBRANE PROTEIN
Release Date:2015-04-22
Deposition Date:2015-02-18
Revision Date:2015-05-06#2015-05-13#2015-05-27#2017-09-06#2017-11-22#2018-02-14
Molecular Weight:47679.23
Macromolecule Type:Protein
Residue Count:414
Atom Site Count:3110
DOI:10.2210/pdb4yay/pdb
Abstract:
Angiotensin II type 1 receptor (AT(1)R) is a G protein-coupled receptor that serves as a primary regulator for blood pressure maintenance. Although several anti-hypertensive drugs have been developed as AT(1)R blockers (ARBs), the structural basis for AT(1)R ligand-binding and regulation has remained elusive, mostly due to the difficulties of growing high-quality crystals for structure determination using synchrotron radiation. By applying the recently developed method of serial femtosecond crystallography at an X-ray free-electron laser, we successfully determined the room-temperature crystal structure of the human AT(1)R in complex with its selective antagonist ZD7155 at 2.9-Å resolution. The AT(1)R-ZD7155 complex structure revealed key structural features of AT(1)R and critical interactions for ZD7155 binding. Docking simulations of the clinically used ARBs into the AT(1)R structure further elucidated both the common and distinct binding modes for these anti-hypertensive drugs. Our results thereby provide fundamental insights into AT(1)R structure-function relationship and structure-based drug design.
| Date made available | 2015 |
|---|---|
| Publisher | RCSB-PDB |