4RWD : XFEL structure of the human delta opioid receptor in complex with a bifunctional peptide

  • Vsevolod Katritch (Contributor)
  • Raymond C. Stevens (Contributor)
  • Bryan L. Roth (Contributor)
  • Gustavo Fenalti (Contributor)
  • Nadia A. Zatsepin (Contributor)
  • Cecilia Betti (Contributor)
  • Patrick Giguere (Contributor)
  • Gye Won Han (Contributor)
  • Andrii Ishchenko (Contributor)
  • Wei Liu (Scripps Research Institute) (Contributor)
  • Karel Guillemyn (Contributor)
  • Haitao Zhang (Contributor)
  • Daniel James (Contributor)
  • Dingjie Wang (Contributor)
  • Uwe Weierstall (Contributor)
  • John C.H. Spence (Contributor)
  • Sébastien Boutet (Contributor)
  • Marc Messerschmidt (Contributor)
  • Garth J. Williams (Contributor)
  • Cornelius Gati (Contributor)
  • Oleksandr M. Yefanov (Contributor)
  • Thomas A. White (Contributor)
  • Dominik Oberthür (Contributor)
  • Markus Metz (Contributor)
  • Chun Hong Yoon (Contributor)
  • Anton Barty (Contributor)
  • Henry N. Chapman (Contributor)
  • Shibom Basu (Contributor)
  • Jesse Coe (Contributor)
  • Chelsie E. Conrad (Contributor)
  • Raimund Fromme (Contributor)
  • Petra Fromme (Contributor)
  • Dirk Tourwé (Contributor)
  • Peter W. Schiller (Contributor)
  • Steven Ballet (Contributor)
  • Vadim Cherezov (Contributor)



Experimental Technique/Method:X-RAY DIFFRACTION
Release Date:2015-01-14
Deposition Date:2014-12-02
Revision Date:2015-04-01#2017-06-07#2018-02-14
Molecular Weight:95688.02
Macromolecule Type:Protein
Residue Count:832
Atom Site Count:6299

Bifunctional μ- and δ-opioid receptor (OR) ligands are potential therapeutic alternatives, with diminished side effects, to alkaloid opiate analgesics. We solved the structure of human δ-OR bound to the bifunctional δ-OR antagonist and μ-OR agonist tetrapeptide H-Dmt-Tic-Phe-Phe-NH2 (DIPP-NH2) by serial femtosecond crystallography, revealing a cis-peptide bond between H-Dmt and Tic. The observed receptor-peptide interactions are critical for understanding of the pharmacological profiles of opioid peptides and for development of improved analgesics.
Date made available2015

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