4EA3 : Structure of the N/OFQ Opioid Receptor in Complex with a Peptide Mimetic

  • Raymond C. Stevens (Contributor)
  • Girolamo Calo (Contributor)
  • Aaron A. Thompson (Contributor)
  • Wei Liu (Scripps Research Institute) (Contributor)
  • Eugene Chun (Contributor)
  • Vsevolod Katritch (Contributor)
  • Huixian Wu (Contributor)
  • Eyal Vardy (Contributor)
  • Xi Ping Huang (Contributor)
  • Claudio Trapella (Contributor)
  • Remo Guerrini (Contributor)
  • Bryan L. Roth (Contributor)
  • Vadim Cherezov (Contributor)



Experimental Technique/Method:X-RAY DIFFRACTION
Release Date:2012-04-25
Deposition Date:2012-03-22
Revision Date:2012-05-30#2017-07-26#2017-11-15
Molecular Weight:98018.35
Macromolecule Type:Protein
Residue Count:868
Atom Site Count:5122

Members of the opioid receptor family of G-protein-coupled receptors (GPCRs) are found throughout the peripheral and central nervous system, where they have key roles in nociception and analgesia. Unlike the 'classical' opioid receptors, δ, κ and μ (δ-OR, κ-OR and μ-OR), which were delineated by pharmacological criteria in the 1970s and 1980s, the nociceptin/orphanin FQ (N/OFQ) peptide receptor (NOP, also known as ORL-1) was discovered relatively recently by molecular cloning and characterization of an orphan GPCR. Although it shares high sequence similarity with classical opioid GPCR subtypes (∼60%), NOP has a markedly distinct pharmacology, featuring activation by the endogenous peptide N/OFQ, and unique selectivity for exogenous ligands. Here we report the crystal structure of human NOP, solved in complex with the peptide mimetic antagonist compound-24 (C-24) (ref. 4), revealing atomic details of ligand-receptor recognition and selectivity. Compound-24 mimics the first four amino-terminal residues of the NOP-selective peptide antagonist UFP-101, a close derivative of N/OFQ, and provides important clues to the binding of these peptides. The X-ray structure also shows substantial conformational differences in the pocket regions between NOP and the classical opioid receptors κ (ref. 5) and μ (ref. 6), and these are probably due to a small number of residues that vary between these receptors. The NOP-compound-24 structure explains the divergent selectivity profile of NOP and provides a new structural template for the design of NOP ligands.
Date made available2012

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