4CAS : Serial femtosecond crystallography structure of a photosynthetic reaction center

  • Linda C. Johansson (Contributor)
  • David Arnlund (Contributor)
  • Gergely Katona (Contributor)
  • Thomas A. White (Contributor)
  • Anton Barty (Contributor)
  • D. P. Deponte (Contributor)
  • Robert L. Shoeman (Contributor)
  • Cecilia Wickstrand (Contributor)
  • Amit Sharma (Contributor)
  • Garth J. Williams (Contributor)
  • Andrew Aquila (Contributor)
  • Michael J. Bogan (Contributor)
  • Carl Caleman (Contributor)
  • Jan Davidsson (Contributor)
  • R. B. Doak (Contributor)
  • Matthias Frank (Contributor)
  • Raimund Fromme (Contributor)
  • Lorenzo Galli (Contributor)
  • Ingo Grotjohann (Contributor)
  • Mark S. Hunter (Contributor)
  • Stephan Kassemeyer (Contributor)
  • Richard Kirian (Contributor)
  • Christopher Kupitz (Contributor)
  • Mengning Liang (Contributor)
  • Lukas Lomb (Contributor)
  • Erik Malmerberg (Contributor)
  • Andrew V. Martin (Contributor)
  • Marc Messerschmidt (Contributor)
  • Karol Nass (Contributor)
  • Lars Redecke (Contributor)
  • M. Marvin Seibert (Contributor)
  • Jennie Sjöhamn (Contributor)
  • Jan Steinbrener (Contributor)
  • Francesco Stellato (Contributor)
  • Dingjie Wang (Contributor)
  • Weixiao Y. Wahlgren (Contributor)
  • Uwe Weierstall (Contributor)
  • Sebastian Westenhoff (Contributor)
  • Nadia A. Zatsepin (Contributor)
  • Sébastien Boutet (Contributor)
  • John C.H. Spence (Contributor)
  • Ilme Schlichting (Contributor)
  • Henry N. Chapman (Contributor)
  • Petra Fromme (Contributor)
  • Richard Neutze (Contributor)



Experimental Technique/Method:X-RAY DIFFRACTION
Release Date:2013-12-25
Deposition Date:2013-10-09
Revision Date:2014-01-08#2017-06-14#2017-11-15
Molecular Weight:146379.91
Macromolecule Type:Protein
Residue Count:1212
Atom Site Count:9890

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 Å resolution and determine its serial femtosecond crystallography structure to 3.5 Å resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.
Date made available2013

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